The dataset shown represents a timelapse sequence showing cell and tissue dynamics during primitive streak formation in chick embryo. The data were acquired using Myr-GFP embryos and a custom built light-sheet microscope. The sequence shows the developmental period covering the time interval from the freshly laid egg (stage EGXIII), prior to the onset of tissue movement until the end of the formation of the primitive streak (Stage HH4). Shown here is only one of the x,y planes from a 3D stack of 100 x,y,z planes that was used for further quantitative analysis of cell and tissue dynamics. The individual results of these analyses are presented as the overlays and are described in the Channel boxes and the accompanying '.txt' file.

The dataset was acquired using a custom light-sheet microscope designed and constructed at the University of Dundee in collaboration between Dr Michael McDonald and Prof. Cornelis Weijer. This work was funded by Biotechnology and Biological Sciences Research Council.

The images can be viewed directly on this website using OMERO.web viewer or downloaded and opened using any program that supports the '.tif' format. To view the sequence with the overlays we recommend to open the '.pattern' file with Bioformats or Fiji with the Bioformats importer installed.

The dataset accompanies data presented in the Nature Cell Biology manuscript 10.1038/ncb3138.
The sample preparation protocol is described in Nature Protocol Exchange 10.1038/protex.2015.012.
The details of the acquisition method will be made available at later time.

The data are hosted by the College of Life Scienices, University of Dundee resources and uses OMERO technology provided by Open Microscopy Environment and Glencoe Software Inc. to store, manage and display the figures.

The dataset is licenced under the Creative Commons Attribution-NonCommercial-NoDerivs License.

Data viewable on this webstie is avaible for download.

Download File:
Zip content description:
channels_descriptions.txt Contains short description of the overlays.
Exp186.patten Opens full dataset with Bioformats or Fiji.
Exp186_T???_C???.tif Single tif Images containing data.